Fig. 8

Subcellular distribution of C2α and C2β in HASM. a Confocal images of subcellular distribution of GFP-C2α and mCherry-C2β. Cells were co-transfected with the expression vectors for GFP-C2α and mCherry-C2β. GFP-C2α is distributed at diffuse fine puncta and perinuclear coarse puncta. mCherry-C2β (mCh-C2β) is distributed at perinuclear coarse puncta and plasma membrane. mCherry-C2β is also distributed diffusely in the mesh-like pattern with some co-localization with GFP-C2α (arrowheads in the lower right panel). b Co-localization of GFP-C2α and mCherry-C2β with clathrin. Cells were co-transfected with the expression vectors of GFP-C2α and mCherry-C2β, and subjected to anti-clathrin heavy chain immunofluorescence staining. Nuclei were stained with DAPI. PI3K- C2α and PI3K-C2β were co-localized with clathrin heavy chain diffusely and at the perinuclear region although the co-localization of PI3K-C2β and clathrin heavy chain was less frequent