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Fig. 6 | The Journal of Physiological Sciences

Fig. 6

From: Class II phosphatidylinositol 3-kinase α and β isoforms are required for vascular smooth muscle Rho activation, contraction and blood pressure regulation in mice

Fig. 6

Attenuated contraction of C2α- and C2β-depleted HASM. a Knockdown of C2α and C2β proteins in HASM by the specific siRNAs. HASMs were transfected with control-, C2α- and C2β-siRNAs and subjected to Western blot analyses for C2α and C2β protein expression. Left, Western blotting. Middle and right panels, quantified data of C2α and C2β protein expression. b Attenuated contraction of C2α- and C2β-double depleted HASM. IMC (0.3 µM)-induced contraction was determined as in Fig. 5. A portion of control siRNA-transfected HASM were pretreated or unpretreated with Y-27632 (10 µM) for 5 min and stimulated with ionomycin for 10 min. Representative images of cells (left) and quantified data (right) are shown. p < 0.05 and p < 0.001 in the indicated comparisons. c No difference in ionomycin-induced increases in the [Ca2+]i in HASM transfected with control-, C2α- and C2β-siRNAs. Left, time-dependent changes in the [Ca2+]i in ionomycin-stimulated cells. Right, quantified data of the peak response of the [Ca2+]i

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