Fig. 2

26S proteasome influence human BM-MSCs proliferation under different hypoxia levels. a Human BM-MSCs proliferation was measured using WST1 Quick assay; the results showed a remarkable decline in the cells proliferation after treating the cells with MG132 inhibitor and severe hypoxia compared to normoxia and moderate hypoxia where it remained unaffected. n = 7; b the doubling time was assessed using trypan blue viability assay. The results depicted a significant rise in the doubling time of human BM-MSCs incubated with MG132 and severe hypoxia weighed against normoxia and moderate hypoxia. However, in moderate hypoxia, the doubling time of human BM-MSCs fell within the normal range. n = 6. c EDU staining for proliferation was performed. The percentage of positive Dapi/EDU purple cells conspicuously high normoxia and moderate hypoxia and less in human BM-MSCs incubated with MG132 and severe hypoxia; n = 3. *p < 0.05 compared to normoxia group; #p < 0.05 compared to moderate hypoxia group. Each experiment was repeated 3 times