Fig. 1
Hypoxia effect on 26S proteasome function in human BM-MSCs: Human BM-MSCs were incubated in hypoxia chamber under different hypoxia levels (0.5–15% O2). a 26S proteasome activity was assessed by measuring fluorescence intensity (RFU) of breaking down 26S specific substrate (Succ-LLVY-AMC), which showed a significant decrease in 26S proteasome activity in severe hypoxia (0.5–1% O2) compared to normoxia and moderate hypoxia (2.5–15% O2), whereas 26S proteasome function remained stable in human BM-MSCs under moderate hypoxia (2.5–15% O2); n = 5. b Western blot detection of 20S proteasome protein levels showed a significant reduction in 20S proteasome protein levels with the gradual raise in hypoxia severity; n = 3.*p < 0.05 compared to normoxia group; #p < 0.05 compared to moderate hypoxia groups. Each experiment was repeated 3 times