Fig. 4

The protective role of lenti-CGRP on high-glucose-triggered ROS and oxidation-related factors. a Representative fluorescence images of intracellular ROS. The upper images are fluorescence images and the lower images are bright field images as controls. The relative fluorescence values were measured by Image-Pro Plus 6.0 software and are expressed as the fold change compared to the 25 mM-treated control group. The data are shown as the mean ± SEM, *p < 0.05. Scale bar = 50 μm. b, c The mRNA expression of eNOS and iNOS under high glucose conditions on days 1, 3, and 5. The data are shown as the mean ± SEM, *p < 0.05. d A western blotting image of MnSOD (SOD2) expression. The data are shown as the mean ± SEM, *p < 0.05