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Fig. 1 | The Journal of Physiological Sciences

Fig. 1

From: Overexpression of calcitonin gene-related peptide protects mouse cerebral microvascular endothelial cells from high-glucose-induced damage via ERK/HIF-1/VEGF signaling

Fig. 1

High glucose exposure decreased the expression of the main CGRP receptor, and the successful lentiviral transduction led to the stable overexpression of CGRP in b.END3 cells. a Immunofluorescence staining of the main CGRP receptor Ramp1 under high glucose for 3 days. Scale bar = 50 μm. b The mRNA expression of Ramp1 under 25 mM and 50 mM glucose conditions after high glucose treatment for 3 days. c The mRNA expression of the negative control group, empty vector-transfected group and lenti-CGRP-transfected group. The negative control group refers to the group that did not undergo any treatment or transduction. d Representative western blotting images of CGRP protein expression in the negative control group, empty vector-transfected group and lenti-CGRP-transfected group. GAPDH was used as a reference protein control on the same membrane. All data are shown as the mean ± SEM, *p < 0.05

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