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Fig. 5 | The Journal of Physiological Sciences

Fig. 5

From: Neuromuscular stimulation ameliorates ischemia-induced walking impairment in the rat claudication model

Fig. 5

Fiber type distribution following hind-limb ischemia (Isch) and effects of exercise training (EX) and electrical stimulation (ES). a–h Representative micrographs of frozen cross sections of myosin heavy chain (MHC) isoforms in the tibialis anterior (TA) oxidative core from the Sham (a, b), Isch (c, d), Isch + EX (e, f), and Isch + ES (g, h) groups. Blue, MHC I; Green, MHC IIa; No staining/black, MHC IIx; Red, MHC IIb. Scale bar, 100 μm. i Quantification of the fiber type distribution in each group. The fiber type distribution was analyzed as the percentage of each fiber type to the total counted fibers. The number of each fiber was counted in 4 fields per rat. n = 5 rats per group. Values are presented as mean ± SE, and statistical significance was determined using Dunnett’s test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Sham group. #P < 0.05, ###P < 0.001, vs. Isch group

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