Fig. 1
Severe EAE increases Tom20, but not Tom70, protein abundance in the mitochondria of the mouse kidney cortex. a Mild EAE does not significantly increase the mitochondrial protein abundance of either Tom20 or Tom70 in the mouse renal cortex. b Severe EAE increases the mitochondrial protein abundance of Tom20 in the extracts from the same region without significantly affecting the mitochondrial protein abundance of Tom70. EAE was induced by subcutaneous injection of MOG35-55 and scored as described in “Methods”. The kidney mitochondrial proteins were extracted by homogenization followed by multiple centrifugations (see “Methods”). The extracts were separated in a 4–12% Bis–Tris gel (Invitrogen) and probed with the rabbit anti Tom20 and Tom70 antibodies. c Neither severe nor mild EAE significantly changes Tom20 mRNA levels. The total RNA from the renal cortex was extracted by ice-cold RNAzol RT kit (Molecular Research Center). Tom20 mRNA abundance was measured by SYBR-based qPCR. (Data are expressed as mean ± SE in this figure and following figures as well. *p < 0.05 vs control; control n = 6, mild EAE n = 5, severe EAE n = 6; unpaired t-test for a, b. One-way ANOVA for c)