Fig. 4

Behavioral parameters and hippocampal nNOS, Akt, and CREB protein expression and phosphorylation levels after 6 weeks of HFD consumption. Open (a) and total arm entries (b), percentage of open/total arm entries (c), and defecation (d). Representative immunoblots are shown in e, and quantified data are presented as nNOS (f), pnNOS^Ser1412/GAPDH (g), pnNOS^Ser1412/nNOS (h), nNOS gene expression levels (i), pAkt^Ser473 (j), Akt (k), pCREB^Ser133 (l), and CREB (m). There were no significant between-group differences after 6 weeks of HFD consumption. Open bars, SD group and light gray bars, HFD group. **P < 0.01, vs SD group. All data are presented as mean ± SE. Dot plot represents individual data points. n = 5–6 per group