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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Mitofusin-2 regulates inflammation-mediated mouse neuroblastoma N2a cells dysfunction and endoplasmic reticulum stress via the Yap-Hippo pathway

Fig. 4

The Mfn2–Yap signaling pathway modulates endoplasmic reticulum stress in N2a cells subjected to TNFα treatment. a–d Proteins were isolated from N2a cells and the expressions of the ER stress proteins PERK, CHOP and GRP78 were measured by western blotting. siRNA against Yap (si-Yap) and adenovirus-loaded Mfn2 (Ad-Mfn2) were transfected into N2a cells. e, f Immunofluorescence assay was used to observe the changes in caspase-12 in N2a cells. Mfn2 overexpression attenuated caspase-12 activity and this effect could be negated by Yap deletion. g ELISA assay was used to verify the activity of caspase-12 in response to Yap deletion and/or Mfn2 overexpression. Data are presented as the mean of three replications with the SEM. Asterisk above columns linked by horizontal line indicates a significant difference between treatments at p < 0.05. Cont Control

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