Fig. 3

Yap is activated by Mfn2 and contributes to the survival of mouse neuroblastoma N2a cells under TNFα treatment. a Cell viability was measured by the MTT assay. siRNA against Yap (si-Yap) was transfected into N2a cells to repress Yap expression. b, c Cellular death was measured by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The number of TUNEL-positive cells was evaluated to reflect the role of Yap in TNFα-mediated N2a cell death. d–f Proteins were isolated from N2a cells and then western blotting was used to observe the changes in caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP). siRNA against Yap was transfected into N2a cells to repress Yap expression. Data are presented as the mean of three replications with the SEM. Asterisk above columns linked by horizontal line indicates a significant difference between treatments at p < 0.05. Pro pro-form of caspase-3, Cle. cleaved, Cont control