Fig. 3

Effect of PDZRN3 on [³H]nicotinate transport activity via SMCT1. To examine the functional change of [³H]nicotinate uptake via SMCT1 by co-expression with PDZRN3, HEK293 cells transfected with SMCT1 wild type (SMCT1wt) or a mutant of SMCT1 that lacks the last three amino acids (SMCT1d3) were incubated with an uptake solution containing 5 nM of [³H]nicotinate for 1 min with or without co-transfection of PDZRN3. Then their radioactivity was measured. Each value represents the mean ± SEM, n = 3. Statistically significant [³H]nicotinate uptake was determined by Student’s t test or one-way ANOVA followed by Fisher’s PLSD (*p < 0.05)