Fig. 2

Co-localization of SMCT1 and PDZRN3. Representative images of HEK293 cells transiently transfected with GFP-fused SMCT1 on the amino terminus and PDZRN3 tagged with FLAG on the carboxyl terminus. HEK293 cells were immunostained with a rabbit anti-GFP antibody and mouse anti-FLAG antibody followed by a goat anti-rabbit IgG conjugated with Alexa Flour 488 (green) and goat anti-mouse IgG conjugated with CF 555 (red) or stained only by secondary antibodies without primary antibodies. The nucleus was stained by DAPI (blue), n = 2