Fig. 1

Interaction between SMCT1 and PDZRN3 through the PDZ binding motif. HEK293 cells were transfected with FLAG-tagged pCMV6-entry or FLAG-tagged pCMV6-entry encoding the full length of PDZRN3. Cell lysates of these cells were co-immunoprecipitated with an anti-FLAG antibody (a) or with an anti-SMCT1 antibody (b). After SDS-PAGE, immunoblot analyses were performed with an anti-SMCT1 antibody (a) or with an anti-PDZRN3 antibody (b). HEK293 cells were transfected with PDZRN3 and pEGFP, GFP-fused SMCT1wt, or GFP-fused SMCT1d3, which is a mutant lacking the carboxyl terminal 3 amino acids from SMCT1. Cell lysates were co-immunoprecipitated with the anti-GFP antibody and immunoblotted with the anti-PDZRN3 antibody (c), n = 2