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Table 1 Primer sets for cloning of TRPM3α and γ variants

From: Identification and classification of a new TRPM3 variant (γ subtype)

 

Forward(5′–3′)

Reverse(5′–3′)

TRPM3α for 1st amplification

GAGAGCTGAGCGCAGGCTG

TCCTGCAACACACGGTAAGCC

TRPM3α for 2nd amplification of target cDNA

ATGGGCAAGAAGTGGAGGGATG

TTAGTTGTGCTTGCTTTCAAAGC

TRPM3γ for 1st amplification

CCAGGAAGCCTCTGCCCTAA

AACTGCTTGCTGCCGGCTTA

TRPM3γ for 2nd amplification of target cDNA

ATGCCAGGGCCGTGGGGGAC

TTATACTGAATAAAAAGGATGTTCTGC

TRPM3α+β+γ for real-time PCR

CGGCCATCATGGCCTGCTAC

GGCAGAACCGGCCTCTCG

TRPM3α for real-time PCR

CTCTGACCGCGAGGACAGCA

GCAGTCCCGAGCGCTTGGTG

TRPM3γ for real-time PCR

CTTATACTGAATAAAAAGGATGTTCTGCAG

CTCTGACCGCGAGGACAGCA

β-actin for real-time PCR

TGTTACCAACTGGGACGACA

AAGGAAGGCTGGAAAAGAGC