Fig. 3
From: Identification and classification of a new TRPM3 variant (γ subtype)
[Ca2+]i increases induced by pregnenolone sulfate or nifedipine treatment of HEK293T cells expressing mouse TRPM3γ or TRPM3α variants. a, b Average changes in [Ca2+]i induced by pregnenolone sulfate (PS, a) or nifedipine (b) treatment of HEK293T cells expressing TRPM3α2 or TRPM3γ2. Cell viability was confirmed by application of 5 μM ionomycin (Iono). The y-axis shows the fura-2 ratio of 340 nm/380 nm. Each symbol represents mean ± SEM of 51–108 cells. c, d Effect of 3, 10, or 50 μM PS (c) and 10, 30, or 100 μM nifedipine (d) on HEK293T cells expressing mouse TRPM3α2, TRPM3α3, TRPM3γ2, or TRPM3γ3. Mock shows results for vector-transfected HEK293T cells. Y-axis: the Δ ratio normalized to ionomycin responses. Each column represents the mean + SEM of 80–280 cells. Statistical significance was assessed using ANOVA followed by a two-tailed multiple t test with Bonferroni correction. **P < 0.01 versus TRPM3α2, ##P < 0.01 versus TRPM3α3