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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Suramin protects hepatocytes from LPS-induced apoptosis by regulating mitochondrial stress and inactivating the JNK-Mst1 signaling pathway

Fig. 4

LPS-induced hepatocyte apoptosis is inhibited by suramin by reducing Mst1 expression. a–e Western blotting was used to detect the alterations in the levels of mitochondrial apoptotic proteins. Hepatocytes were treated with LPS and/or suramin. Meanwhile, Mst1 adenovirus (Ad-Mst1) was used to reverse Mst1 expression. f, g Mitochondrial ROS production was determined using flow cytometry. h–j The concentrations of cellular antioxidants were measured using ELISAs. Hepatocytes were treated with LPS and/or suramin. Meanwhile, Mst1 adenovirus (Ad-Mst1) was used to reverse Mst1 expression. k, l Immunofluorescence staining for liberated cyt-c. DAPI was used to label the nucleus, and the nuclear cyt-c expression was measured to reflect the cyt-c liberation into the cytoplasm/nucleus. Hepatocytes were treated with LPS and/or suramin. Meanwhile, Mst1 adenovirus (Ad-Mst1) was used to reverse Mst1 expression. Ad-Mst1 Mst1 adenovirus, Ad-ctrl control adenovirus *p < 0.05

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