Fig. 2

Mitochondrial bioenergetics are regulated by suramin via repressing Mst1 expression. a ATP production was measured to reflect cellular metabolism in the setting of LPS-induced inflammation. b, c The mitochondrial membrane potential was determined using the JC-1 probe. The relative mitochondrial membrane potential was calculated by measuring the ratio of red/green fluorescence intensities. d–g ELISA was used to observe the alterations in components of the mitochondrial respiratory complex in response to LPS treatment. h Glucose uptake assay using hepatocytes challenged with LPS. i Lactic acid production was evaluated using an ELISA. Ad-Mst1 Mst1 adenovirus, Ad-ctrl control adenovirus, *p < 0.05