Fig. 1

Suramin attenuates LPS-mediated hepatocyte death by reducing Mst1 expression. a, b LPS was incubated with hepatocytes for 12 h, and different doses of suramin were concomitantly administered to hepatocytes. Then, western blotting was used to observe Mst1 levels. c Cell viability was determined using the MTT assay following the LPS treatment and suramin application. d, e The Mst1 adenovirus (Ad-Mst1) was transfected into hepatocytes, and then Mst1 levels were measured using western blotting. f An LDH release assay was used to observe cell death in response to suramin in cells with or without Mst1 overexpression. g, h TUNEL staining was used to detect apoptotic hepatocytes. The number of TUNEL-positive cells was observed following the suramin treatment and/or Mst1 overexpression. i Caspase-3 activity assay. Hepatocytes were treated with LPS and/or suramin. Meanwhile, Mst1 adenovirus (Ad-Mst1) was used to reverse Mst1 expression. Ad-Mst1 Mst1 adenovirus, Ad-ctrl control adenovirus, *p < 0.05