Fig. 3

Representative protein expression of eNOS, VEGF, MDM-2, and TSP-1 by Western blot (a). Mean protein expression levels of eNOS (b), VEGF (c), MDM-2 (d), and TSP-1 (f) in the soleus muscles in each group. The data are expressed as fold change (a.u.) from the value in the CON group, which is set to a value of 1. The levels of protein expression were normalized to GAPDH levels. Values are presented as mean ± SEM. * and ^† indicate significantly different from CON with same intervention and CO₂ (−) vs. CO₂ (+), respectively, at p < 0.05