Fig. 5

FUNDC1-related mitophagy sustains mitochondrial dysfunction and blocks I/R-mediated mitochondrial apoptosis. a ATP production was measured via ELISA in response to FUNDC1 deficiency and Mst1 deletion. b Caspase-3 activity was measured after I/R injury in cardiomyocytes transfected with FUNDC1 siRNA. c–h After H/R injury in vitro, proteins were isolated and western blotting was performed to analyze the expression of proteins related to mitochondria. i, j TUNEL assay for cellular apoptosis. Green nuclei indicate apoptotic cells, and the number of TUNEL-positive cells was quantified. Data are presented as the mean ± SEM. H/R injury hypoxia–reoxygenation injury, WT-cell cardiomyocytes isolated from WT mice, Mst1-KO cell cardiomyocytes isolated from Mst1 knockout mice. *P < 0.05