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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Mst1 promotes cardiac ischemia–reperfusion injury by inhibiting the ERK-CREB pathway and repressing FUNDC1-mediated mitophagy

Fig. 4

Mst1 deletion reverses FUNDC1-related mitophagy in the context of cardiac I/R injury. a–g After H/R injury in vitro, proteins were isolated and mitophagy activity was monitored via western blotting. Moreover, to verify whether mitophagy activity was predominately regulated by FUNDC1, we transfected an siRNA into Mst1-deleted cardiomyocytes. The transfection efficiency was confirmed via western blotting. After loss of FUNDC1 in Mst1-deleted mice, mitophagy activity was re-examined via western blotting. h, i Mitophagic activity was further validated via immunofluorescent staining of mitochondria and lysosomes. The fusion of mitochondria (green fluorescence) and lysosomes (red fluorescence) indicates mitophagy, which is indicated by orange fluorescence. Data are presented as the mean ± SEM. H/R injury hypoxia–reoxygenation injury, WT-cell cardiomyocytes isolated from WT mice, Mst1-KO cell cardiomyocytes isolated from Mst1 knockout mice. *P < 0.05

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