Fig. 5

Whole-cell experiments for channel function of Δ(G970-T1122)-CFTR proteins. Representative whole-cell current traces obtained from CHO cells expressing WT-, C-terminal FLAG-tagged WT-, Δ(G970-T1122)-, and C-terminal FLAG-tagged Δ(G970-T1122)-CFTR proteins. CFTR currents were stimulated by 10 μM forskolin, and 5 μM CFTRinh-172 (inh-172) was subsequently applied to confirm the CFTR currents. The lower panel shows the I–V relationships of forskolin-induced conductance by subtracting the leak currents at position I from the overall current at position II. Each group represents the average of five data points