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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Characterization of Δ(G970-T1122)-CFTR, the most frequent CFTR mutant identified in Japanese cystic fibrosis patients

Fig. 4

Effects of the C-terminal FLAG tag on expression of Δ(G970-T1122)-CFTR proteins. a Immunoblots for WT- and Δ(G970-T1122)-CFTR with or without C-terminal FLAG tag. The black and gray arrowheads indicate mature and immature forms of CFTR proteins. Cells were incubated at 27 °C for 48 h after transfection. b Summary for the immunoblot signal intensity of immature (grey) and mature (black) forms of WT- and Δ(G970-T1122)-CFTR proteins. Data were normalized to the total signal intensity and expressed as relative protein expression levels. Mean value ± SD from triplicate experiments. Statistical significance (*, p < 0.01) was evaluated by Student’s t test. c Immunocytochemical staining of CHO cells transiently expressing C-terminal FLAG-tagged WT- and Δ(G970-T1122)-CFTR proteins with (P) or without (non-P) membrane permeabilization. Red, FLAG-tagged CFTR proteins stained with Alexa Fluor 555-linked anti-FLAG antibody; blue, nuclei (DAPI). Scale bar 10 µm. Cells were incubated at 27 °C for 48 h after transfection (color figure online)

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