Fig. 2
From: MARCKS phosphorylation and amylase release in GLP-1-stimulated acini isolated from rat pancreas
Effect of GLP-1 on MARCKS phosphorylation. a, b MARCKS and p-MARCKS in lysates (20 μg protein) were detected by Western blotting analysis using anti-MARCKS and anti-p-MARCKS antibodies, respectively. a Acini were stimulated with GLP-1 (25 nM) for the indicated times. b Acini were stimulated with 8-bromo-cAMP (1 mM) for 15 min. c, d The Western blotting data for p-MARCKS were normalized against stimulation at 0 min. Values show the means ± SE from three independent experiments. The asterisks indicate a significant difference (P < 0.05) compared with stimulation at 0 min (a) or the control (b)