Fig. 1
From: Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo
The experimental setup. a Insertion and fixation of the microprism: the top view (top) and the side view (bottom). b Attachment of the head chamber on the mouse. c The array of power LEDs on the circuit board. d Schematic drawings of the all-optical system. The LED light was focused in the cortical layer 5 (L5) directly through the dichroic mirror, the objective lens and the microprism with no interference from the two-photon imaging system