Fig. 4

The localization of the basal membrane anion transporter SULP-8 was affected by arf-1.2 RNAi. a–dtmEx3093 [sulp-4p::SULP-4::EGFP] animals were treated with control RNAi (a, b) or the arf-1.2 RNAi (c, d). e–htmEx3096 [sulp-8p::SULP-8::EGFP] animals were treated with control RNAi (e, f) or the arf-1.2 RNAi (g, h). Fluorescence (a, c, e, g) and DIC (b, d, f, h) images. Magnified images are shown in the insets. The arrowheads indicate the basal membrane of the excretory canals. Scale bars = 20 µm. SULP-4::EGFP and SULP-8::EGFP localized at the apical membrane and basal membrane, respectively. Intracellular vacuoles with multi-compartments were labeled by SULP-8::EGFP but not by SULP-4::EGFP