Fig. 3

The subcellular localization of ARF-1.2 in the excretory canals. a, b The wild-type ARF-1.2 displayed cytosolic distribution with a partially punctate pattern. c, d The constitutively active form ARF-1.2(Q71L) exhibited a punctate pattern. No vacuoles were observed. e, f The dominant negative form ARF-1.2(T31N) showed cytosolic distribution in the excretory canals. The arrowheads indicate vacuoles. Young adult tmEx3104 (for the wild type), tmEx3137 (for QL form), and tmEx3317 (for TN form) hermaphrodites were photographed. Scale bars = 20 µm. g Alignment of the Homo sapiens (Hs) ARF1, C. elegans (Ce) ARF-1.2 and Saccharomyces cerevisiae (Sc) Arf1p. The numbers indicate the positions of amino acid residues. Fully conserved amino acid residues are indicated by a reverse background, and similar but not identical residues are shaded