Fig. 1

arf-1.2 RNAi affects the morphology of the excretory cell. a–dtmIs807 [hmit-1.2p::GFP] animals expressing GFP in the excretory cell (P0) were treated by control RNAi (a, b) or the arf-1.2 RNAi (c, d). L2 hermaphrodites (F1) were photographed. Fluorescence (a, c) and DIC (b, d) images. The arrows indicate the canal tubes. The canals of the arf-1.2-treated animals were thinner (c) than those of the control animals (a). The arrowheads indicate vacuoles in the excretory canals (c). e, f Magnified fluorescence (e) and DIC (f) images of the vacuole observed in the arf-1.2 RNAi. The arrows indicate the lumen. Besides a major vacuole (asterisk), small vacuoles (arrowheads) were observed in the photographed animal. Scale bars = 10 µm. (g) The percentages of animals that displayed abnormal morphology of the excretory canals. (h–k) tmIs806 [hmit-1.2p::GFP] animals expressing GFP in the excretory cell. Wild-type animals (h, i) or the arf-1.2 mutants (j, k). Fluorescence (h, j) and DIC (i, k) images. Adult hermaphrodites were photographed. Scale bars = 50 µm