Fig. 6
From: Hydroxyhydroquinone impairs fat utilization in mice by reducing nitric oxide availability
Effect of HHQ and L-NAME on the oxygen consumption rate (OCR) in primary mouse hepatocytes. The energy metabolism of the hepatocytes was measured using an XF96 Extracellular Flux Analyzer. The cells were treated with either HHQ (10 or 20 µM; a and b) or L-NAME (LN; 100 or 200 µM; c and d) 18 h before the assay (a and c). At the start of the assay, either sodium palmitate (Pal) or BSA was added, and the time-course of changes in the OCR was measured (b and d). The incremental or decremental area under the curve (AUC) was calculated according to the trapezoid rule. Values are the mean ± SEM of N = 7 – 9 wells/group. a #: ; < 0.05, control-Pal vs HHQ 20 µM-Pal. c *P < 0.05, **P < 0.01, control-Pal vs L-NAME 100 µM-Pal; ##: P < 0.01, ###: P < 0.001, control-Pal vs L-NAME 200 µM-Pal (Dunnett’s post hoc test). b, d Lower-case letters above the bars represent significant differences between the treatment groups (P < 0.05, one-way ANOVA followed by Bonferroni’s post hoc test). The P value for the trend (Ptrend) was calculated using the Jonckheere trend test