Skip to main content

Table 2 Biometrical analysis, adipose tissue, adipose depot mass, and biochemical and hormonal determinations in adult male and female rats raised in small and normal litters (PND60, n = 8/group)

From: Litter size reduction accentuates maternal care and alters behavioral and physiological phenotypes in rat adult offspring

 

Litter size

Normal litter

Small litter

Male

Female

Male

Female

Biometrical analysis

 Body weight

261.3 ± 2.81

149.5 ± 2.29

321.6 ± 6.36***

177.5 ± 1.77***

 Lee index

3.06 ± 0.01

2.84 ± 0.04

3.27 ± 0.01***

3.00 ± 0.02**

Adipose tissue (g/100 g b.w.)

 Retroperitoneal fat

0.61 ± 0.02

0.90 ± 0.06

1.07 ± 0.05***

1.58 ± 0.17***

 Epididymal fat

0.72 ± 0.01

 

0.97 ± 0.03***

 

 Ovarian fat

 

1.04 ± 0.07

 

2.06 ± 0.19***

Biochemical and hormonal determinations

 Glucose (mg/dl)

105.00 ± 0.93

97.87 ± 2.40

118.00 ± 2.73**

113.20 ± 4.07**

 Triglycerides (mg/dl)

135.70 ± 11.70

73.21 ± 11.55

169.80 ± 10.61*

109.30 ± 10.24*

 Total cholesterol (mg/dl)

70.93 ± 3.71

70.88 ± 3.40

85.37 ± 4.12*

89.48 ± 7.84*

 HDL-C (mg/dl)

4.38 ± 0.14

4.67 ± 0.29

3.32 ± 0.10***

3.69 ± 0.15**

 Insulin (ng/ml)

0.52 ± 0.04

0.29 ± 0.01

0.56 ± 0.07

0.41 ± 0.04*

 Leptin (ng/dl)

0.69 ± 0.10

0.70 ± 0.06

1.92 ± 0.25**

1.67 ± 0.05***

  1. Values are the mean ± SEM (Student t-test)
  2. *p < 0.05, **p < 0.01; ***p < 0.001 compared with the normal litter