Fig. 5

Modulation of the expression of Kv7.1, CREB and Sp1 by dihydrotestosterone (DHT) or isoproterenol (ISO). Cardiomyocytes were incubated with DHT (3 nM) or ISO (0.1 µM) for 24 h in the presence or absence of a PKA inhibitor H89 (1 µM) to assess the expression of Kv7.1 (a), CREB (b), and Sp1 (c). Modulation of Kv7.1 expression by DHT (3 nM) was assessed with or without an Sp1 specific inhibitor mithramycin (50 nM) (d). Data are expressed as the mean ± SEM. *p < 0.05 vs. control, ^#p < 0.05 vs ISO