Fig. 4
Short-term (5 min) and long-term (24 h) effect of dihydrotestosterone (DHT) on IKs. Representative IKs traces before (control) and 5 min after DHT (3 nM) treatment (a). IKs was activated by depolarizing voltage steps indicated in inset. E-4031 (5 µM) and 4AP (4 mM) were given to the bath solution to minimize the interference of IKr and Ito, respectively. Representative IKs traces from ventricular cardiomyocytes cultured for 24 h without DHT (vehicle) and with 3 nM DHT (b). Group data of current (I)–voltage (V) relationship of IKs for the short-term effect (c) and long-term effect of DHT (d). Data are presented as the mean value ± SEM (n = 6). *p < 0.01, **p < 0.05, p = 0.052 vs vehicle