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Fig. 2 | The Journal of Physiological Sciences

Fig. 2

From: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease

Fig. 2

Expression level and immunofluorescence analysis of substantia nigral and striatal α-syn in MPTP mice after treadmill exercise. Representative western blot showing α-syn levels in the substantia nigra (SN) for all groups of mice are presented. a, b α-Syn was increased in MPTP mice compared with C57BL mice and the levels of α-syn in the SN of MPTP mice were reduced after treadmill exercise. β-actin was probed as an internal control. Fluorescence photomicrographs of 40-μm-thick SN and striatal sections from all groups of mice immunolabeled with anti-α-syn antibody. c Note that immunofluorescence labeling reveals that α-syn expression was notably enhanced in SN and striatal region in MPTP mice whereas the enhanced expression was suppressed after treadmill exercise. d, e Number of α-syn-positive neurons in SN and STR region. Values are expressed to 100% for levels of C57BL mice. Six mice per C57BL mice and MPTP mice subgroups were assayed in triplicate in western blot analysis and four mice per C57BL mice and MPTP mice subgroups were assayed in triplicate in immunofluorescence assay. Scale bars: 100 μm. LSD post hoc test after ANOVA. Values are mean ± SEM. Saline control (CON), MPTP treatment with probenecid (MPTP), and MPTP treatment with probenecid plus treadmill exercise (MPTP-TE)

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