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Table 1 Effects of hypergravity and vestibular lesion (VL) on the mRNA levels of humoral factors linking muscle to bone in the soleus muscle

From: Effects of hypergravity on gene levels in anti-gravity muscle and bone through the vestibular system in mice

Gene

2 weeks

8 weeks

Sham-1 g

Sham-2 g

VL-1 g

VL-2 g

Sham-1 g

Sham-2 g

VL-1 g

VL-2 g

IGF-1

1.00 ± 0.11

1.43 ± 0.11

1.07 ± 0.13

1.21 ± 0.16

1.00 ± 0.20

1.25 ± 0.18

1.51 ± 0.23

1.47 ± 0.22

FGF2

1.00 ± 0.16

1.02 ± 0.08

1.30 ± 0.26

1.07 ± 0.10

1.00 ± 0.14

1.07 ± 0.11

0.84 ± 0.05

1.31 ± 0.32

TGF-β1

1.00 ± 0.11

1.66 ± 0.14**

0.80 ± 0.13

1.24 ± 0.14

1.00 ± 0.23

0.83 ± 0.09

0.67 ± 0.07

1.16 ± 0.13

MSTN

1.00 ± 0.28

0.92 ± 0.32

0.76 ± 0.24

1.12 ± 0.51

1.00 ± 0.17

0.77 ± 0.19

0.58 ± 0.25

0.82 ± 0.29

Act A

1.00 ± 0.13

0.87 ± 0.05

0.95 ± 0.14

0.89 ± 0.05

1.00 ± 0.30

1.03 ± 0.09

1.42 ± 0.36

1.16 ± 0.25

FST

1.00 ± 0.12

1.08 ± 0.08

1.21 ± 0.04

1.13 ± 0.07

1.00 ± 0.27

0.75 ± 0.08

1.19 ± 0.18

0.94 ± 0.16

FNDC5

1.00 ± 0.09

1.27 ± 0.07

0.86 ± 0.12

0.99 ± 0.16

1.00 ± 0.09

1.22 ± 0.09

1.06 ± 0.15

1.28 ± 0.18

IL-6

1.00 ± 0.10

1.28 ± 0.25

1.23 ± 0.10

1.14 ± 0.13

1.00 ± 0.20

2.50 ± 0.71

2.28 ± 0.40

1.61 ± 0.61

OGN

1.00 ± 0.12

1.20 ± 0.11

1.12 ± 0.08

1.21 ± 0.38

1.00 ± 0.09

0.77 ± 0.15

0.96 ± 0.08

0.88 ± 0.12

FAM5C

1.00 ± 0.10

0.72 ± 0.08

1.08 ± 0.08

0.93 ± 0.09

1.00 ± 0.70

0.67 ± 0.09

1.13 ± 0.37

0.89 ± 0.35

  1. Total RNA was extracted from the soleus muscle of mice treated with VL or sham surgery after exposure to 1 or 2 g for 2 and 8 weeks. Then, the level of insulin-like growth factor-1 (IGF-1), fibroblast growth factor 2 (FGF2), transforming growth factor-β1 (TGF-β1), myostatin (MSTN), activin A (Act A), follistatin (FST), fibronectin type III domain-containing 5 (FNDC5), interleukin-6 (IL-6), osteoglycin (OGN), family with sequence similarity 5, member C (FAM5C) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was assessed by real-time PCR analysis. Data are expressed relative to the levels of GAPDH. The data represent the mean ± SEM of 6 (2 weeks) and 4 (8 weeks) tissues in each group.
  2. ** P < 0.01. vs. sham-1 g
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The Journal of Physiological Sciences

ISSN: 1880-6562

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