Fig. 7
Effects of cytosolic calcium on single-channel currents activated by a hypotonic solution. a Average traces of fura-2 ratio changes in hTRPA1-expressing cells pre-loaded with BAPTA-AM or treated with thapsigargin, and the effects of hypotonic stimulus (−120 mOsm) in the absence of extracellular Ca2+. Data are from cells with (N = 47) or without (N = 116) 10 μM BAPTA-AM. b, c Representative traces of hTRPA1-mediated single-channel currents in HEK293T cells exposed to 1 μM thapsigargin (3 min) before hypotonic stimulus in the absence of extracellular Ca2+ without (b) or with (c) preloading with 10 μM BAPTA-AM. d Comparison of hTRPA1-mediated single-channel currents activated by hypotonic solutions with (N = 11) or without (N = 17) pre-exposure to 1 μM thapsigargin and single-channel currents in BAPTA-AM pre-loaded cells (N = 11)