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Fig. 1 | The Journal of Physiological Sciences

Fig. 1

From: Hypotonicity-induced cell swelling activates TRPA1

Fig. 1

Hypotonic solutions induced an increase in cytosolic calcium concentrations in cells expressing human TRPA1. a Changes in cytosolic Ca2+ concentrations with time. Data are presented as the fura-2 ratio (340/380 nm) in HEK293T cells expressing human TRPA1(A1) or cells transfected with pcDNA3.1 (Mock) (N = 72) in the presence of extracellular Ca2+ after 1 min exposure to hypotonic solutions with (N = 93) or without (N = 93) 1 μM AITC. b Hypotonic solutions increased cytosolic Ca2+ concentrations. Comparison of the fura-2 ratio changes at the end of each solution application. §§ p < 0.01 vs ±0 mOsm in the mock-transfected cells. *p < 0.05; **p < 0.01 vs 0 mOsm in hTRPA1-transfected cells without 1 μM AITC. †† p < 0.01 vs 0 mOsm in hTRPA1-transfected cells with 1 μM AITC

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