Fig. 3

Daily expression profiles of clock genes Per2 and Bmal1, clock-controlled gene Dbp, and aquaporin-5 (Aqp5) mRNA in the SMGs of Cry1 ^−/− Cry2 ^−/− mice collected in vivo. a–d Per2 (a), Bmal1 (b), Dbp (c), and Aqp5 (d) mRNA expression levels in the SMGs of WT mice (black columns) and Cry1 ^−/− Cry2 ^−/− mice (white columns). The black and white horizontal bar at the top of each graph indicates the dark and light phases, respectively. Data are shown as mean ± standard error of the mean (SEM) (n = 6 WT mice per time point and n = 4 Cry1 ^−/− Cry2 ^−/− mice per time point). Significance of the analysis of variance: *p < 0.05, **p < 0.01. ZT Zeitgeber time