Fig. 3

The potentiating effect of CGRP on the TRPV1 activity evoked by capsaicin. Trigeminal neurons were incubated with CGRP at various doses (10 nM, 100 nM or 1 µM) or control treatment for various times (1, 4, and 24 h) before wash-out of the medium and recording of the reproducible inward currents evoked by 10 µM capsaicin. a The inward current responses of the trigeminal neurons evoked by 10 µM capsaicin (arrow). b The histogram summarizes the current densities (pA/pF). The results are expressed as the mean ± SEM. Statistics was performed using Bartlett’s tests and ANOVA with Tukey–Kramer tests. *p < 0.05 and **p < 0.01 compared with the control; n = 6 for 1 and 24 h, and n = 8 for 4 h