Fig. 2

The effect of capsaicin on CaMKII phosphorylation (pCaMKII) in CGRP-incubated TG organ cultures. After incubation with 1 µM CGRP for 1 h, the TG organ cultures were subjected to 30 min capsaicin (3 µM) challenges. The control cultures were incubated with culture medium for 90 min. Some cultures were incubated with 1 µM CGRP for 90 min or 3 µM capsaicin for 30 min. The proteins were isolated from the TG organ cultures and subjected to western blot analysis for pCaMKII (50 kDa), and the amounts were quantified (relative to β-actin). The results are expressed as the mean ± SEM of four independent experiments. Statistics was performed using ANOVA with Tukey–Kramer tests. *p < 0.05 and ***p < 0.01 compared with the control, ^# p < 0.05 compared with the capsaicin and ^## p < 0.01 compared with the CGRP plus capsaicin