Fig. 4

Real-time PCR analysis of Fbxo32 and Trim63 mRNA expression, respectively, in EDL muscle normalized to Gapdh. Data are expressed as mean ± SEM (n = 8–10 animals/group), in arbitrary units (AU). In white Fed:Control and Fasting:Control and in black Fed:HMB and Fasting:HMB; double asterisks vs. Fed (p < 0.001); in (a) asterisk vs. Fed:Control and HMB (p < 0.05) and in (b) asterisk vs. Fed:Control (p < 0.05)