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Fig. 3 | The Journal of Physiological Sciences

Fig. 3

From: Sphingosine-1-phosphate pretreatment amends hypoxia-induced metabolic dysfunction and impairment of myogenic potential in differentiating C2C12 myoblasts by stimulating viability, calcium homeostasis and energy generation

Fig. 3

S1P mediates temporal secretion of myokines in hypoxic differentiating C2C12 cells. Media supernatant from S1P (1 μM) and VC pretreated differentiating C2C12 cells under normoxia (N) and 0.5% hypoxia (H) at various time points was analyzed for the composition of secreted myokines—IL-6 (a), IFN-γ (b) and TNF-α (c); 0.2 × 105/cm2 C2C12 cells were seeded and induced to differentiate as described. Following this the media supernatant was snap-frozen for myokine content analysis using a sandwich ELISA-based commercial kit. Data are reported as mean ± SD from three independent experiments carried out in triplicate. Statistical analysis was performed using the one-way ANOVA/post hoc Bonferroni’s analysis method. Statistical significance is denoted by NVC vs. HVC ($p < 0.05, *p ≤ 0.005) and HVC vs. HS1P (@p < 0.05, #p ≤ 0.005) at respective time points

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