Fig. 2
Morphometric analysis of terminally differentiated myotubes: 0.2 × 105/cm2 C2C12 myoblast cells were seeded and induced for myotube formation as described previously. Morphometric analysis was done in calcein-AM stain differentiated C2C12 myotubes (>600 µm length) at 8D in normoxia VC and S1P pretreated cells (8NVC vs. 8NS1P). a Number of myotubes/field was counted manually. b Myotube length was measured by using ImageJ software. c Myotube diameter was measured by using ImageJ software. d Number of branches (Y shaped structures) was counted manually. Data are reported as mean ± SD from three independent experiments carried out in triplicate. Statistical analysis was performed using unpaired Student's t test. Statistical significance is denoted by NVC vs. NS1P (**p < 0.05, *p ≤ 0.005)