Fig. 5
From: Epac activation inhibits IL-6-induced cardiac myocyte dysfunction
Effects of Epac activation on Ca2+ concentration (a, b) and contractility of cardiac myocytes (c, d). a Intracellular Ca2+ concentration in response to increasing concentrations of isoproterenol (ISO; 0–10−5 mol/L) in adult rat cardiac myocytes with or without a 6-h IL-6 (30 ng/ml) pretreatment. b The effect of a 5-h 8-CPT-AM (10 μmol/L) pretreatment on the ISO-induced increase of Ca2+. Pretreatment with 8-CPT-AM restored the increase of Ca2+ transients suppressed by IL-6 (n = 29–64, **significantly different at p < 0.01, two-way ANOVA). c Cardiac cell shortening at baseline and in response to ISO (10−5 mol/L). d Pretreatment with 8-CPT-AM (10 μM) restored cell shortening suppressed by IL-6 (30 ng/ml) (n = 24–31, ****significantly different at p < 0.0001, two-way ANOVA). Data are presented as the mean ± SEM