Fig. 3
From: Epac activation inhibits IL-6-induced cardiac myocyte dysfunction
Time course of Epac-mediated expression of suppressor of cytokine signaling-3 and -1 (SOCS3, SOCS1, respectively) in cardiac myocytes. a, b mRNA expression of SOCS3 (a) and SOCS1 (b) was examined by quantitative real-time PCR in neonatal rat cardiac myocytes in the presence of 8-CPT-AM (10 μmol/L) for the indicated time. mRNA expression of SOCS3 and SOCS1 gradually increased with 8-CPT-AM treatment. a SOCS3 mRNA expression peaked at a significant increase of 1.7-fold at 1 h after 8-CPT-AM treatment (n = 4, *significantly different at p < 0.05 vs. baseline, one-way ANOVA). b SOCS1 mRNA expression peaked at a significant increase of 6.2-fold at 3 h after 8-CPT-AM treatment (n = 3–4, **significantly different at p < 0.01 vs. baseline, one-way ANOVA). NS Not significant. Data are presented as the mean ± SEM