Fig. 7

EPA did not stimulate Mn²⁺ entry. a bEND.3 cells in Ca²⁺-containing solution were excited with 360 nm, treated with 1 mM Mn²⁺ and subsequently DMSO, 30 μM EPA or 5 μM ionomycin. There are significant differences (P < 0.05) between DMSO-EPA beginning at 180 s and afterwards, and between DMSO-ionomycin beginning at 164 s and afterwards. b The same protocol as in (a) in which 100 μM EPA and respective DMSO control were added. There are significant differences (P < 0.05) between DMSO-EPA between 159 and 569 s, and between DMSO-ionomycin beginning at 164 s and afterwards. The data are normalized with the initial fluorescence reading. Results are mean ± SEM of 28–51 cells from 3 to 5 separate experiments