Fig. 2

Inhibitory effect of siRNA-driven p130Cas knockdown on collagen gel contraction. a Western blot analysis with NHDFs transfected with a siRNA specific for p130Cas (p130Cas siRNA) or with a scrambled non-specific siRNA (control siRNA) before and 4 days after gel contraction. b Inhibition of contraction of NHDF-embedded collagen gel by knockdown of p130Cas expression. The surface areas of the collagen gels were measured and the relative contraction ratios were assessed. Data are mean ± SD (n = 3 per group). c Significant inhibition of contraction of skin models of siRNA-treated NHEK- and NHDF-embedded collagen gels. Two days after release from the well walls, photographs of the skin models were taken, followed by measurement of their sizes. Data are mean ± SD (n = 6 per group). *p < 0.05, **p < 0.01