Fig. 5
From: Origin of oligodendrocytes in mammalian forebrains: a revised perspective
In utero electroporation. Neural precursor cells in the dorsal cortex (a) or lateral and medial dorsoventral boundary (b) were labeled by electroporating E15.5 Z/EG reporter mouse embryos with a Cre recombinase expression plasmid. GFP+ cells were traced in coronal sections of P30 mouse brains and classified as oligodendrocytes (arrows), astrocytes (arrowheads), or neurons (not shown) based on morphology. c Suppression of Wnt signaling by the expression of Dkk caused neural precursor cells in the dorsal cortex to also differentiate into oligodendrocytes (arrows). Scale bars 50 μm (modified from Naruse et al. [10])