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Fig. 2 | The Journal of Physiological Sciences

Fig. 2

From: Origin of oligodendrocytes in mammalian forebrains: a revised perspective

Fig. 2

Lineage tracing using Cre recombinase transgenic/knock-in mice. a Nestin-CreER;Z/EG and Olig2 CreER;Z/EG embryos were labeled by injection of 2 mg 4-hydroxytamoxifen (4OHT) intraperitoneally into the dam at E11.5, then cells from the germinal zone of the ganglionic eminence were used in a colony-forming neurosphere assay at E14.5. Neurospheres with or without GFP expression are indicated by arrows or arrowheads, respectively. Scale bars 200 μm. b Schematic diagram of Nestin- and Olig2-lineage progeny (cells shown in red and blue, respectively) of neural precursor cells labeled by injection with 4OHT (shown as a vertical green line). c Nestin-CreER;Z/EG and Olig2 CreER; Z/EG mice were labeled at the indicated times and brains were analyzed at P30. GFP+ cells in the cortex were classified as oligodendrocytes (O), astrocytes (A), or neurons (N) based on morphology, and the number of cells from each type were counted (modified from Naruse et al. [10])

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