Fig. 5
Design of experimental chambers for muscles. a Experimental chamber (7.5 mm diameter) for 31P NMR studies of contracting frog muscles. A single pair of frog sartorius muscles can be held in a vertical position parallel to the central glass tube by the support system constructed of Teflon, glass and epoxy resin. By these arrangements muscles can be stimulated, force produced can be recorded and the muscles can be superfused with oxygenated Ringer solution. Dawson et al. [22]. b Chamber of 25 mm diameter for 31P NMR studies, in which 8 pairs of semitendinosus muscles of bullfrogs can be held. The use of many muscles (average 4.4 g) greatly improved the signal intensity as is seen from the results shown in Fig. 6. Yamada and Tanokura [115]